Journal: STAR Protocols

Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach

doi: 10.1016/j.xpro.2026.104439

Figure Lengend Snippet: 96-well plate setup for K D probe determination

Article Snippet: 96-well conical black plate , Thermo Fisher ScientificTM , #249945.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach

doi: 10.1016/j.xpro.2026.104439

Figure Lengend Snippet: 96-well plate setup for FASST assay

Article Snippet: 96-well conical black plate , Thermo Fisher ScientificTM , #249945.

Techniques:

Journal: Microsystems & Nanoengineering

Article Title: High-fidelity bioassembly of organoids and spheroids using inertial droplet microfluidics for precision oncology and tumor microenvironment modeling

doi: 10.1038/s41378-026-01244-x

Figure Lengend Snippet: The automated OsciSphere bioassembly platform and workflow. a 3D model of the OsciSphere platform. The inset shows the core 8-channel droplet generator, which integrates an oscillator, syringe pump, disposable pipette tips, and a cooling block to maintain Matrigel in a liquid state. b Photograph of the automated workstation layout. c Schematic of the OsciSphere workflow. 1) Cells are suspended in Matrigel at 4 °C. 2) The platform’s droplet generator dispenses uniform droplets into a 96-well plate containing a biphasic carrier oil/culture medium overlay. 3) Droplets are solidified at 37 °C for 10 min. 4) Solidified microspheres are transferred into the underlying medium using an antistatic gun or gentle agitation. 5) This automated process yields arrays of µMCTs or µTDOs ready for high-throughput screening applications. d Time-lapse imaging (left) demonstrates the “pull-break-sediment” cycle of droplet formation. The platform reliably produces approximately 100 uniform 30 nL droplets from only 3 µL of Matrigel (right), achieving excellent size uniformity. Scale bar, 500 µm. e Encapsulation uniformity demonstrated by fluorescent beads within Matrigel microspheres (60.9 ± 5.1 beads per droplet, CV = 7.67%, n = 28). f Platform versatility is shown by forming uniform microspheres from agarose and HAMA. g Radar chart comparing OsciSphere’s performance metrics (e.g., uniformity, throughput, automation) against conventional 3D culture methods. OsciSphere enables rapid and uniform 3D culture, including ( h ) µMCTs and i µTDOs. j Dome TDOs exhibit significant spatial heterogeneity, leading to diffusion-limited regions and apoptotic cores by Day 3

Article Snippet: Droplets are ejected into receiving containers—either 96-well plates or Eppendorf tubes—prefilled with culture medium containing 0.1% Tetronic 90R4 to prevent droplet coalescence, and overlaid with the carrier oil.

Techniques: Transferring, Blocking Assay, Gentle, High Throughput Screening Assay, Imaging, Encapsulation, Diffusion-based Assay